Publication date: 25th July 2016
Cell migration is a key process in several biological contexts such as in development, cancer and wound healing. Current in vitro migration assays lack in the accurate control of important variables that orchestrate cell motility. We developed a system that allows the control of soluble and insoluble factors, as well as the mechanical cues in a high-throughout apparatus. This is achieved by creating dynamically adhesive micropatterned substrates, which allow the binding of specific ECM-mimetic peptides to the patterned polymer brushes via "click chemistry". We adapted and optimised this micro-fabricated system for standard 96-well plate formats and confirmed that cell migration can be reprodicibly induced and quantified by high-content imaging. To demonstrate the utility of this model, we used a collagen mimetic peptide (GFOGER) to activate human keratinocyte migration in the presence of a panel of 150 chromatin modifying compounds and screened for novel epigenetic regulators of re-epithelialisation. In summary, the high-throughput micro-patterned assay described here represents a novel and tuneable system in which to study cell migration, and on-going studies aim to understand how cross-talk between intrinsic and extrinsic factors regulate keratinocyte migration and wound repair.
nanoGe is a prestigious brand of successful science conferences that are developed along the year in different areas of the world since 2009. Our worldwide conferences cover cutting-edge materials topics like perovskite solar cells, photovoltaics, optoelectronics, solar fuel conversion, surface science, catalysis and two-dimensional materials, among many others.
nanoGe Fall Meeting (NFM) is a multiple symposia conference celebrated yearly and focused on a broad set of topics of advanced materials preparation, their fundamental properties, and their applications, in fields such as renewable energy, photovoltaics, lighting, semiconductor quantum dots, 2-D materials synthesis, charge carriers dynamics, microscopy and spectroscopy semiconductors fundamentals, etc.
This conference is a unique series of symposia focused on advanced materials preparation and fundamental properties and their applications, in fields such as renewable energy (photovoltaics, batteries), lighting, semiconductor quantum dots, 2-D materials synthesis and semiconductors fundamentals, bioimaging, etc.
International Conference on Hybrid and Organic Photovoltaics
International Conference on Hybrid and Organic Photovoltaics (HOPV) is celebrated yearly in May. The main topics are the development, function and modeling of materials and devices for hybrid and organic solar cells. The field is now dominated by perovskite solar cells but also other hybrid technologies, as organic solar cells, quantum dot solar cells, and dye-sensitized solar cells and their integration into devices for photoelectrochemical solar fuel production.
Asia-Pacific International Conference on Perovskite, Organic Photovoltaics and Optoelectronics
The main topics of the Asia-Pacific International Conference on Perovskite, Organic Photovoltaics and Optoelectronics (IPEROP) are discussed every year in Asia-Pacific for gathering the recent advances in the fields of material preparation, modeling and fabrication of perovskite and hybrid and organic materials. Photovoltaic devices are analyzed from fundamental physics and materials properties to a broad set of applications. The conference also covers the developments of perovskite optoelectronics, including light-emitting diodes, lasers, optical devices, nanophotonics, nonlinear optical properties, colloidal nanostructures, photophysics and light-matter coupling.
The International Conference on Perovskite Thin Film Photovoltaics Perovskite Photonics and Optoelectronics (NIPHO) is the best place to hear the latest developments in perovskite solar cells as well as on recent advances in the fields of perovskite light-emitting diodes, lasers, optical devices, nanophotonics, nonlinear optical properties, colloidal nanostructures, photophysics and light-matter coupling.